Dispersion and Chemical Reaction
Single Line Configuration
2.2.6.
This clip demonstrates the speed and efficiency of mixing, promoted by flow reversal in the single interface sample/zone sequence.
The dye used previously to visualize the concentration gradient is an acidobasic indicator, that is blue in basic and yellow in acid form. The sequence shows aspiration of 100 microliters of the dye, followed by 50 microliters of 0.01N HCl, aspirated from the reagent port. Note that during reagent aspiration, the dye retreats into the holding coil, and it is being followed by the reagent. When the flow is reversed, the reacted (yellow) leading edge of the dye enters to flow cell, followed by the tailing section, which is more diluted then front of the reacted zone. Finally the system is flushed with a burst of rapid flow.
Since acid-base reactions are very fast, this experiment documents the speed and efficiency of mixing of sequentially stacked zones by a single flow reversal .
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